Harry Potter Wizards Collection brings home all eight movies on a ridiculous 31 discs (video) originally appeared on Engadget on Sun, 25 Mar 2012 10:54:00 EDT. Please see our terms for use of feeds.
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XBMC Eden officially steps out of beta, available for download now originally appeared on Engadget on Sat, 24 Mar 2012 20:12:00 EDT. Please see our terms for use of feeds.
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Testimony began before the Synod of the Pacific, consisting of ministers and church elders from throughout the West, on a case stemming from a decision by the Community Presbyterian Church of Danville, an affluent San Francisco suburb, to join a more conservative denomination.
A San Francisco-area church governing body agreed in 2010 to allow the Danville congregation to keep real estate that had been appraised at nearly $14 million after its parishioners voted to split from the mainline Presbyterian Church U.S.A.
Critics of the move, however, have argued that the regional governing body lacked authority to approve the property transfer.
"We are here today because we believe the Presbytery of San Francisco has made an unauthorized gift of property," Joan Blackstone, a retired lawyer and church elder who represents opponents of the property transfer, said in opening statements.
Thursday's trial highlights deep divisions within the parent church and its 2 million members, as well as other mainstream Protestant denominations, over the ordination of gay clergy and blessing of same-sex unions.
Although it does not allow ministers to perform same-sex marriages, the Presbyterian Church allows its ministers to bless gay unions.
The synod's trial, held in a hotel banquet room near the San Francisco Airport, will decide whether the presbytery properly used the church's so-called "gracious-dismissal" policy to allow the Danville parish to leave with its buildings and more than 7 acres of land held in trust for the Presbyterian Church U.S.A.
The committee of local ministers "considered, prayed about it and decided the buildings should continue to be used by the Danville congregation as they continue to do God's work," the Rev. Joan Huff, a San Francisco minister who represents the presbytery, told the tribunal. "Grace is not always fair. Grace cannot be monetized. It cannot be bought, earned or won."
"It if could, it would cease to be grace," she said.
Blackstone urged the 10 commissioners hearing the case to consider the impact of their decision on church groups throughout the nation struggling with how to divide property.
OPENED CLERGY TO HOMOSEXUALS
The Presbyterian Church U.S.A. formally opened the ranks of its clergy to homosexuals last spring in a move that triggered a schism, prompting some opposed to gay clergy to form a new denomination, the Evangelical Covenant Order of Presbyterians.
The Presbyterian Church's pension board further alienated some conservative congregants this month by approving benefits for same-gender partners and their children starting next year.
Upset over differences in biblical interpretation of social issues, including questions of gay life and abortion, the Danville congregation began separating from the parent church in 2010.
They want to join yet another offshoot denomination, the Evangelical Presbyterian Church. That church believes "unrepentant homosexual behavior is incompatible with the ordination vows."
"This case is going to establish a precedent of sorts in the minds of people in this denomination," Blackstone said in her opening trial statement. "It's going to be considered by congregations as they request to be dismissed."
The Kentucky-based denomination has endured other splits in its history, including schisms over slavery and the ordination of women. But the Rev. Gradye Parsons, the highest-ranking official in the church general assembly, said some congregations that have left the fold during the past 20 years have rejoined.
"I think it's sad that people are leaving," he said. "I see it more as a separation than a divorce and hope someday we can be reconciled."
The Danville church, founded in 1865, consists of about 1,800 members.
The Rev. Scott Farmer, the church's senior pastor, served on a task force charged with writing the "gracious-dismissal" policy, Blackstone said. She questioned whether Farmer had a conflict of interest when the Danville church was the first to ask to be dismissed under the policy.
Farmer could not be reached for comment.
A website for the Presbyterian Lay Committee, a conservative advocacy group, listed 35 congregations that had started the process of separating from the denomination since July. The First Presbyterian Church in Colorado Springs, with more than 4,000 members, is the largest to take part in the exodus.
The synod is expected to issue a written decision next week.
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Source: http://www.thechurchreport.com/index.cfm?objectID=151371
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Andrew Medichini / AP
Pope Benedict XVI waves as he boards a plane on his way to a six-day visit to Mexico and Cuba, at Rome's Fiumicino international airport, on Friday
By msnbc.com news services
ABOARD THE PAPAL PLANE -- Pope Benedict said on Friday communism no longer works in Cuba and the Catholic Church was ready to help the island find new ways of moving forward without "trauma."
Speaking on the plane taking him from Rome for a trip to Mexico and Cuba, the pope told reporters: "Today it is evident that Marxist ideology in the way it was conceived no longer corresponds to reality."
Responding to a question about his visit to the island, a communist bastion 90 miles off the coast of the United States for more than 50 years, Benedict added: "New models must be found with patience and in a constructive way...we want to help."
Benedict, who is due to arrive in Cuba on Monday after a three-day visit to Mexico, called for freedom of conscience and freedom of religion on the island.
Cuba detains 70 'Ladies in White' ahead of Pope visit
Also on Friday, Benedict promised to "unmask the evil" of drug trafficking in Mexico, which has been wracked by a surge in gang violence over the past five years.
'We are all fed up'
The pope gave a forceful response when asked on board his plane about drug trafficking in Mexico, which has generated violence claiming some 50,000 lives since 2007.
"We must do whatever is possible to combat this destructive evil against humanity and our youth," he told reporters. "It is the responsibility of the Church to educate consciences, to teach moral responsibility and to unmask the evil, to unmask this idolatry of money which enslaves man, to unmask the false promises, the lies, the fraud that is behind drugs."
Car bomb targets Mexico newspaper office
The relentless bloodletting was in the mind of many of those waiting to see Benedict in Leon, a Roman Catholic stronghold that has avoided the worst of the brutal turf wars between drug cartels and clashes with security forces.
"The Church has to address the violence, give us a message that there can be change. We are all fed up, our society has been hurt," said Ruben Santibanez, a local doctor.
Christophe Pierre, the Vatican's diplomatic representative in Mexico, said Benedict would not ignore Mexico's plight.
"Violence is being talked about, we can't hide it, but there is a lot more to Mexico than the violence, and the desire to live in harmony is precisely what the Holy Father is coming for," he said on Thursday.
Images: Mexico gets ready for Pope's visit
Crowds of devout Mexicans readied campsites in Leon in preparation for a massive outdoor mass on Sunday where hundreds of thousands of people are expected.
Benedict hopes to rally the faithful in the world's second most populous Catholic nation as more Mexicans are lured to evangelical Protestant churches.
But the German pope faces a challenge generating the same kind of fervor as his charismatic Polish predecessor, Pope John Paul, who was beloved in Latin America and drew pulsating crowds whenever he criss-crossed the region during his 27-year papacy.
Abuse scandals
Highlighting the growing religious divisions in Mexico, a large Protestant church in Leon held a service with hundreds of worshipers on Thursday, prominently displaying a sign that read "We are not Roman Catholics."
The Vatican has been stung by accusations of child abuse by priests in parishes around the world - including revelations about Mexican religious leader Marcial Maciel, who founded a prominent Catholic order, the Legionaries of Christ.
Maciel, who died in 2008 at the age of 87, was disgraced by allegations of sexual abuse and drug addiction.
Heaven scent: Pope orders eau de cologne that he alone may wear
During the pope's visit, religious scholar Bernardo Barranco will present a new book by ex-Legionaries with scores of leaked Vatican documents the authors say prove the Holy See knew about Maciel's molestation of young boys and morphine use for decades.
The hacker group Anonymous in Mexico crashed at least two of the websites for Benedict's visit to Mexico on Thursday, claiming his trip is a political move to support the president's party.
Anonymous Mexico said in a video posted on social media sites that the pope's visit will cost Mexicans money that could be better spent on the poor, and is meant to support the PAN in the July 1 presidential election. PAN candidate Josefina Vazquez Mota trails front-runner Enrique Pena Nieto of the Institutional Revolutionary Party by at least 10 percentage points in most polls.
Reuters and The Associated Press contributed to this report.
More from msnbc.com and NBC News:
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Source: http://worldnews.msnbc.msn.com/_news/2012/03/23/10829604-pope-says-communism-does-not-work-in-cuba
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The Islamic regime in Tehran was not satisfied with the public support the Iranian Jewish community?s was forced to confer on the Global March to Jerusalem for which Iran is recruiting Islamists worldwide. Now, the event?s organizers, Majlis Speaker Hossein Sheikh-ol-Eslam and Salim Ghafouri, have ordered the community to send a Jewish delegation to march with the Islamist groups in Lebanon, debkafile?s Iranian sources disclose.
The delegations are scheduled to mass on the Lebanese and Jordanian borders with Israel and at West Bank and Gaza checkpoints on March 30, when Israeli Arabs mark Earth Day every year.
Iran?s ancient Jewish community of around 15,000 souls (9,000 in Tehran, 4,000 in Shiraz and 1,300 in Isfahan) has been living in fear of reprisals should Israel or the United States carry out a military operation against the country?s nuclear facilities. ?Now, they face a fresh danger of murder and abduction by Hizballah and Palestinian gunmen and terrorists in Lebanon.
Jewish communal leaders were instructed by the Iranian authorities this week to have at least 10 young men aged 18 to 22 ready for the march. They were to be given ?the honor? of acting as vanguard for breaking through the Lebanese-Israeli border fence and leading a mass incursion across the border.
They suspect that this ploy is meant to prevent Israeli soldiers from firing on the trespassers for fear of killing the Jewish contingent, while at the same time, exposing them to violence when the event is over at the hands of al-Qaeda linked Palestinian groups under Hizballah protection.
The Salafi doctrine held by the al Qaeda killer Muhammad Merah who murdered four Jews, including three children, in Toulouse Monday, is rife in the south Lebanese Palestinian Ain Hilwa refugee camp. The most active are two Palestinian jihadist groups, the Abdullah Azzam Brigades, which now and then shoots rockets into northern Israel, and Jund al-Sham, which is closely tied to al Qaeda branches in Lebanon, Syria and Iraq, where they call themselves Osbat al-Ansar.
The two terrorist groups are the bosses of the Safouri Quarter of the camp.
Our sources report an Iranian scheme to send the Jewish marchers on a visit to Ain Hilwa to show their solidarity with the most radical Palestinian cause.
Last week, Jewish leaders were obliged to sign a declaration of solidarity with the Global March and condemnation of Israel. The text put before them for signing was as follows: We the Jews of Iran strongly condemn the barbaric crimes of the occupation regime in Palestinian and declare the Zionist state in violation of the principles of Our Teacher Moses and the Will of God. We are totally at one with the aspirations of the heroic Palestinian people.?
Signed: Dr. Syamak Mare Dedeq, Jewish Member of Parliament, and Rabbi Mashallah Golestani-Nejad, described as the Chief Rabbi of Iran.
debkafile?s Iranian sources add: Tehran is the main bankroller and live wire of the Global March against Israel?s borders and claims to have rounded up Islamist delegations from five continents to support the Palestinians. Seventy sympathizers are on their way to Lebanon, Syria and Jordan from India, Malaysia, Pakistan and other Asian countries.
To mark the event, Tehran staged a cartoon contest. The winner drew around the Al Aqsa mosque in Jerusalem a wall modeled on the fences of Auschwitz.
Both the organizers are members of the Ministry of Intelligence MOIS with long experience?of managing Iranian activities on behalf of Arab and Palestinian terrorist groups. Sheikh-ol-Islam, while holding the post of Deputy Speaker of Parliament, also coordinates Tehran?s relations with the Lebanese Hizballah.
On Feb. 26, Supreme Leader Ayatollah Ali Khamenei proclaimed the launching of the Global March to Jerusalem an expression of Iran?s policy for strengthening ?resistance operations? against Israel and guarding Palestinian interests.
Source: http://www.debka.com/article/21856/
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Tumor Research
[Abstract]
Prostate cancer is the most common malignancy in men .Both the morbidityand mortality rate from prostate cancer rise almost exponentially in recent years.Many researches showed that its tumorgenesis and biological characters are closelyrelated to many genes. Growing evidence suggests that many kallikreins areimplicated in it. Human kallikrein gene 7(KLK7; HSCCE), also named HSCCE/PRSS6, is a new member of the human kallikrein gene family, which was found inskin first, had drawn great attention and been undertaken wide studies in manymalignant tumors.Objective The aim of this study were : (1) to determine whether KLK7 gene isexpressed in prostate cell line PC-3. (2) to investigate whether DHT had theeffects on KLK7 mRNA level in PC-3 cell in vitroMethods (1) Total RNA was extracted from human prostate cell line PC-3 andhuman kallirein7(KLK7) gene cDNA was amplified by PCR after reverse-transcription using Oligo (dT) primer. Amplified products was ligated to PGEM-TEasy plasmid vector. The recombinant plasmid was transfered to the competentE.coli DH5?. Indentified by enzyme digestion, KLK7 gene in recombinant plasmidwas sequenced by sanger dideoxy-mediated chain-termination method. (2)Thehuman prostate cell line PC-3 were cultured at 37?in a CO2 incubator with F12culture medium and were divided into seven groups by different DHT stimulatingconcentrations of 0M?10-9M?10-8M?10-7M?10-6M?10-5M and 0.01% Ethanol.Following culture in day 1, day 2, day 3 and day 4, MTT method was applied toobserve the cell proliferation . RT-PCR was engaged to find the KLK7 level in thePC-3 cell under the different concentrations of DHT stimulation.Results (1)The cloned kallikrein7 gene is about 762 bp in length. A sequencecomparison of this with the reported kallikrein7 sequence in the Genebankindicated that the cloned prostate cell line PC-3 KLK7 gene has two nucleotidedifference. (2) In day 1 of culture, the cell treated with 10-8mol/l DHT grew fast,there was significant increase of OD in this group compared with the control group( P < 0.05). In day 4 of culture , there were significant increase of OD in thegroups treated with 10-9mol/l?10-8mol/l?10-7mol/l?10-6mol/l DHT compared withthe control group ( P < 0.05) except the group treated with 10-5mol/l DHT (P >0.05). (3) RT-PCR showed that the group treated with 10-9mol/L ?10-8mol/L?10-7mol/L?10-6mol/L DHT had no KLK7 mRNA expression. TheKLK7 mRNA OD value in control group and 10-5mol/L DHT group was 0.9061?0.9367 respectively compared with control gene .Conclusion Our study indicated: (1) KLK7 gene is expressed in prostate cellline. It may be provide the evidences for further studies of the KLK7 gene inprostate cancer. (2) Lower concentration of DHT can enhance the proliferation ofPC-3 cell. Its optimal concentration is 10-8mol/L. (3) the expression of KLK7mRNA may be regulated by androgens and has certain relations with prostatecancer cell growth. Further studies are required to prove this hypothesis.
Title: Molecular Cloning of Human Prostate Cancer Cell Line KLK7 Gene and the Effects of DHT on KLK7 mRNA Level in PC-3 Cell in Vitro
Category: Malignant Tumor
Filename: Molecular Cloning of Human Prostate Cancer Cell Line KLK7 Gene and the Effects of DHT on KLK7 mRNA Level in PC-3 Cell in Vitro.pdf
Pages: 128
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Source: http://www.tumorres.com/malignant-tumor/41928.htm
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